Some nonpathogenic microorganisms can stimulate plant immune responses without
damaging the plants, which allows them to act like plant vaccines, but screening microorganisms for such properties has traditionally been time-consuming and expensive.
Associate Professor Toshiki Furuya and Professor Kazuyuki Kuchitsu of Tokyo University of Science and their colleagues decided to develop a screening strategy involving cultured
plant cells. A description of their method appears in a paper recently published in Scientific Reports.
The first step in this screening strategy involves incubating the candidate microorganism together with BY-2 cells, which are tobacco plant cells known for their rapid and stable growth rates. The next step is to treat the BY-2 cells with cryptogein, which is a protein secreted by fungus-like pathogenic microorganisms that can elicit immune responses from tobacco plants.
A key part of the cryptogein-induced immune responses is the production of a class of chemicals called reactive oxygen species (ROS), and scientists can easily measure cryptogein-induced ROS production and use it as a metric for evaluating the effects of the nonpathogenic microorganisms.
To put it simply, an effective pretreatment agent will increase the BY-2 cells’ ROS production levels (i.e., cause the cells to exhibit stronger immune system activation) in response to cryptogein exposure.
To test the practicability of their screening strategy, Dr. Furuya and his colleagues used the strategy on 29 bacterial strains isolated from the interior of the Japanese mustard spinach plant (Brassica rapa var. perviridis), and they found that 8 strains boosted cryptogein-induced ROS production.
They then further tested those 8 strains by applying them to the root tips of seedlings from the Arabidopsis genus, which contains species commonly used as model organisms for studies of plant biology. Interestingly, 2 of the 8 tested strains induced whole-plant resistance to bacterial pathogens.
Based on the proof-of-concept findings concerning those 2 bacterial strains, Dr Furuya proudly notes that his team’s screening method “can streamline the acquisition of microorganisms that activate the immune system of plants.”
When asked how he envisions the screening method affecting agricultural practices, he explains that he expects his team’s screening system “to be a technology that contributes to the practical application and spread of microbial alternatives to chemical pesticides.”
In time, the novel screening method developed by Dr Furuya and team may make it significantly easier for crop scientists to create greener agricultural methods that rely on the defence mechanisms that plants themselves have evolved over millions of years.